fpg

formamidopyrimidine-DNA glycosylase
BBF10K_002805
source
Mycoplasma pneumoniae M129

Involved in base excision repair of DNA damaged byoxidation or by mutagenic agents. Acts as DNA glycosylasethat recognizes and removes damaged bases

attr.
Keoni Gandall

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-fpg
plasmid size
2896
insert size
834
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.