K12polLF (exo)

DNA Polymerase I, Large (Klenow) Fragment (3′→5′ exo-)
BBF10K_003249
source
Escherichia coli (strain K12)

DNA polymerase fragment used in flourescent labelling for microarray, dA and dT tailling, and important in the process of ligating DNA adapters to DNA fragments. Frequently used in prepararing DNA libraries for Next-Gen sequencing. The exo-Klenow polymerase is a mutant polymerase from the Klenow DNA polymerase large fragment that lacks 5'-3' polymerase activity and 3'-5' exonuclease activity."

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-K12polLF (exo)
plasmid size
3353
insert size
1230
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Freemont, P.S., Ollis, D.L., Steitz, T.A. and Joyce, C.M., 1986. A domain of the Klenow fragment of Escherichia coli DNA polymerase I has polymerase but no exonuclease activity. Proteins: Structure, Function, and Bioinformatics, 1(1), pp.66-73.

intellectual property

We are unaware of third-party property rights claims on uses of this item.