ECOpolA

E. coli DNA Polymerase I
BBF10K_003250
source
Escherichia coli (strain K12)

DNA polymerase that (in addition to polymerase activity) exhibits 3'-5' and 5'-3' exonuclease activity. Able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-ECOpolA
plasmid size
4910
insert size
2787
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Lehman, I.R., Bessman, M.J., Simms, E.S. and Kornberg, A., 1958. Enzymatic synthesis of deoxyribonucleic acid I. Preparation of substrates and partial purification of an enzyme from Escherichia coli. Journal of Biological Chemistry, 233(1), pp.163-170.

intellectual property

We are unaware of third-party property rights claims on uses of this item.