BstpolLF

Bst DNA Polymerase, Large Fragment
BBF10K_003262
source
Bacillus stearothermophilus

Fragment retains 5´ → 3´ polymerase activity from full length Bst DNA Polymerase, while lacking 5´ →3´ exonuclease activity. Suitable for applications requiring thermophilic strand displacement. Additionally used for Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template. Can be used in the standard sanger one step or two step protocol which separates the labelling reaction from the enlongation termination reaction. The enzyme can be used in double stranded sequencing.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-BstpolLF
plasmid size
3893
insert size
1770
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

McClary, J., Ye, S. Y., Hong, G. F., & Witney, F. (1991). Sequencing with the large fragment of DNA polymerase I from Bacillus stearothermophilus. DNA Sequence, 1(3), 173-180. Aliotta, J. M., Pelletier, J. J., Ware, J. L., Moran, L. S., Benner, J. S., & Kong, H. (1996). Thermostable Bst DNA polymerase I lacks a 3′→ 5′ proofreading exonuclease activity. Genetic analysis: biomolecular engineering, 12(5-6), 185-195.

intellectual property

We are unaware of third-party property rights claims on uses of this item.