BstpolLFBst DNA Polymerase, Large Fragment
Fragment retains 5´ → 3´ polymerase activity from full length Bst DNA Polymerase, while lacking 5´ →3´ exonuclease activity. Suitable for applications requiring thermophilic strand displacement. Additionally used for Loop-Mediated Isothermal Amplification (LAMP), DNA sequencing through high GC regions, rapid sequencing from nanogram amounts of DNA template. Can be used in the standard sanger one step or two step protocol which separates the labelling reaction from the enlongation termination reaction. The enzyme can be used in double stranded sequencing.
Chiara Gandini, Open Bioeconomy Lab
Escherichia coli Top10
37 C, shaking 300 rpm
3' - AATG … GCTT - 5'
ColE1 High Copy
other informationNo Value