T7gene5

T7 DNA Polymerase (unmodified)
BBF10K_003268
source
Escherichia phage T7 (Bacteriophage T7)

DNA Polymerase that catalyzes the replication of T7 phage DNA during infection. The protein dimer has two catalytic activities: DNA polymerase activity and strong 3´→ 5´ exonuclease (1,2,3). The high fidelity and rapid extension rate of the enzyme make it particularly useful in copying long stretches of DNA template.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T7gene5
plasmid size
4238
insert size
2115
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Mark, D. F., & Richardson, C. C. (1976). Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase. Proceedings of the National Academy of Sciences, 73(3), 780-784.

intellectual property

We are unaware of third-party property rights claims on uses of this item.