Pfulig

Pfu DNA Ligase
BBF10K_003275
source
Pyrococcus furiosus

DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair. Catalyzes template-dependent ligation at temperatures of about 30° C. to about 80° C., and substantially retains its catalytic activity when subjected to temperatures of about 85° C. to about 100° C.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Pfulig
plasmid size
3809
insert size
1686
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Sillero, M. A. G., Montes, M., de Diego, A., del Valle, M., Atencia, E., & Sillero, A. (2002). Thermostable Pyrococcus furiosus DNA ligase catalyzes the synthesis of (di) nucleoside polyphosphates. Extremophiles, 6(1), 45-50.

intellectual property

We are unaware of third-party property rights claims on uses of this item.