EcoRIR

EcoRI restriction enzyme
BBF10K_003281
source
Escherichia coli RY13

Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-EcoRIR
plasmid size
2957
insert size
834
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Modrich, P. A. U. L., & Zabel, D. O. N. N. A. (1976). EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme. Journal of Biological Chemistry, 251(19), 5866-5874.

intellectual property

We are unaware of third-party property rights claims on uses of this item.