PstIR

PstI restriction enzyme
BBF10K_003283
source
Providencia stuartii 164

Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-PstIR
plasmid size
3104
insert size
981
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Armstrong, Karen, and William R. Bauer. "Preferential site-dependent cleavage by restriction endonuclease PstI." Nucleic acids research 10.3 (1982): 993-1007.

intellectual property

We are unaware of third-party property rights claims on uses of this item.