MMLV_RT (mut H)

Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
BBF10K_003285
source
Moloney Murine Leukemia Virus

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-MMLV_RT (mut H)
plasmid size
4109
insert size
1986
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Kotewicz, M.L., D'Alessio, J.M., Driftmier, K.M., Blodgett, K.P. and Gerard, G.F., 1985. Cloning and overexpression of Moloney murine leukemia virus reverse transcriptase in Escherichia coli. Gene, 35(3), pp.249-258. This variant is published in: Liu, S., Goff, S. P., & Gao, G. (2006). Gln84 of moloney murine leukemia virus reverse transcriptase regulates the incorporation rates of ribonucleotides and deoxyribonucleotides. FEBS letters, 580(5), 1497-1501.

intellectual property

We are unaware of third-party property rights claims on uses of this item.