MMLV_RT (lack H)

Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase RNaseH - (lacking RNaseH domain) (SuperScriptII)
BBF10K_003286
source
Moloney Murine Leukemia Virus

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

attr.
Chiara Gandini, Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-MMLV_RT (lack H)
plasmid size
3635
insert size
1512
origin
ColE1 High Copy
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Shinnick, Thomas M., Richard A. Lerner, and J. Gregor Sutcliffe. "Nucleotide sequence of Moloney murine leukaemia virus." Nature 293.5833 (1981): 543-548.

intellectual property

We are unaware of third-party property rights claims on uses of this item.