Eco31IB

Eco31IB methyltransferase
BBF10K_003294
source
No Value

Site-specific DNA methyltransferase 2; protects DNA from cleavage by Eco31I

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Eco31IB
plasmid size
3314
insert size
1212
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Bitinaite, J., et al. "Evolutionary relationship of Alw26I, Eco31I and Esp3I, restriction endonucleases that recognise overlapping sequences." Molecular Genetics and Genomics 267.5 (2002): 664-672.

intellectual property

We are unaware of third-party property rights claims on uses of this item.