SapIM1

M1.SapI methyltransferase
BBF10K_003296
source
Saccharopolyspora sp.

Site-specific DNA methyltransferase 1; protects DNA from cleavage by SapI

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-SapIM1
plasmid size
3254
insert size
1153
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Xu, S-Y., et al. "Cloning and expression of the Apa LI, Nsp I, Nsp HI, Sac I, Sca I, and Sap I restriction-modification systems in Escherichia coli." Molecular and General Genetics MGG 260.2-3 (1998): 226-231.

intellectual property

We are unaware of third-party property rights claims on uses of this item.