Bst HF

Bst polymerase, High fidelity
BBF10K_003311
source
Bacillus stearothermophilus

High fidelity Bst. It has an error rate of about 7.8x10^-7, a fidelity about 20 times higher than standard Bst, and better than Phusion in High-GC buffer. The specifically patented enzyme is the polymerase, which is a NEB proprietary strand displacing polymerase with high fidelity. Luckily Bst-HF is precisely that, a high-fidelity strand displacing polymerase. The kit is supposed to run at 50°C (-60°C), which also happens to be within the optimal temperature range for Bst.One other use for Bst-HF, or even better fusion variants on a Taq scaffold of that enzyme, would be for high-GC sequences. The addition of a bit of thermotolerant strand displacing polymerase lets one amplify DNA sequences with well over 80% GC with ease, and boosts amplification of large fragments due to its‘ ability to overcome complex/problematic sequences

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Bst HF
plasmid size
3893
insert size
1791
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

N/A

intellectual property

We are unaware of third-party property rights claims on uses of this item.