TthligK294R

Tth DNA Ligase K294R
BBF10K_003314
source
Thermus thermophilus

High fidelity version Taq DNA Ligase

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-TthligK294R
plasmid size
4154
insert size
2052
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.