DNA Topoisomerase 1B Vaccinia Virus

DNA Topoisomerase 1B Vaccinia Virus
BBF10K_003324
source
No Value

Used in TOPO cloning. Recognizes the DNA sequence 5´-(C/T)CCTT-3' and digests double stranded DNA at this sequence. The energy from this breakage is stored as a covalent bond between the DNA and the topoisomerase. If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent bond thus joining the two DNA strands and releasing topoisomerase.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-DNA Topoisomerase 1B Vaccinia Virus
plasmid size
3068
insert size
966
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

https://www.ncbi.nlm.nih.gov/pubmed/2541548 (1989), first partial isolation here https://www.ncbi.nlm.nih.gov/pubmed/17115 (1977)

intellectual property

We are unaware of third-party property rights claims on uses of this item.