lambda red operon

lambda red operon (exo, bet, gam)
BBF10K_003325
source
No Value

Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA (including E. coli genome), electroporate a linear donor DNA substrate (either dsDNA or ssDNA) into E. coli expressing the lambda red enzymes. These enzymes then catalyze the homologous recombination of the substrate with the target DNA sequence (mutagenesis uccurs in vivo). ~50 nucleotides of homology are required to the target site for recombination.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-lambda red operon
plasmid size
4273
insert size
2171
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.