Exonuclease III E. coli

Exonuclease III E. coli
BBF10K_003330
source
Escherichia coli

Double-stranded DNA specific exonuclease. Initiates at the 3' termini of linear double-stranded DNA with 5' overhangs or blunt ends and 3' overhangs containing less than four bases. Initiates at nicked sites in double-stranded DNA. Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3' to 5' direction. Ideal for site-directed mutagenesis, preparation of single-stranded DNA for dideoxy sequencing, preparation of nested deletions in double-stranded DNA

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Exonuclease III E. coli
plasmid size
2930
insert size
807
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

from E. coli genome, published in 1988 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC211488/

intellectual property

We are unaware of third-party property rights claims on uses of this item.