Firefly Luciferase [Photinus pyralis] (Fluc)

Firefly Luciferase [Photinus pyralis] (Fluc)
BBF10K_003368
source
Photinus pyralis

luciferin 4-monooxygenase from common eastern firefly. Produces green light with a wavelength of 562 nm. Most commonly used luciferase reporter.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Firefly Luciferase [Photinus pyralis] (Fluc)
plasmid size
3776
insert size
1653
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli. Proc Natl Acad Sci U S A. 1985 Dec;82(23):7870-3. https://www.ncbi.nlm.nih.gov/pubmed/3906652

intellectual property

We are unaware of third-party property rights claims on uses of this item.