Luciferase [Luciola cruciata] (Japanese firefly)

Luciferase [Luciola cruciata] (Japanese firefly)
BBF10K_003369
source
Gaussia princeps/Luciola cruciata?

Luciferase from Gaussia princeps. At 19kD, it is the smallest known luciferase and its catalytic properties make it one of the “brightest” known. Additionally, multiple disulfide bonds contribute to the protein stability at elevated temperatures. The first report of in vivo imaging using Gluc demonstrated sensitivity that is several orders of magnitude higher than the first generation luciferases, Firefly (Fluc) and Renilla lucifereses

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Luciferase [Luciola cruciata] (Japanese firefly)
plasmid size
3770
insert size
1647
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.