Renilla luciferase

Renilla luciferase
BBF10K_003370
source
Renilla reniformis

36kDa protein from sea pansy (Renilla reniformis). It produces a blue light of 480nm, and it requires only coelenterazine and oxygen. It can be used in dual-reporter assays with Firefly luciferase due to their differences in substrate requirements and light output.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-Renilla luciferase
plasmid size
3059
insert size
936
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

citations

Isolation and expression of a cDNA encoding Renilla reniformis luciferase. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4438-42. https://www.ncbi.nlm.nih.gov/pubmed/1674607

intellectual property

We are unaware of third-party property rights claims on uses of this item.