BC_BCD2_His_fuGFP_TEVcut

BC_BCD2_His_fuGFP_TEVcut
BBF10K_003420
source
N/A

A BCD combined with a his N terminal purification tag, free use GFP, and a TEV cleavage site. Defined as a BC part.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - TACT … AATG - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-BC_BCD2_His_fuGFP_TEVcut
plasmid size
2997
insert size
877
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.