CE_T7RNApol_Stop

CE_T7RNApol_Stop
BBF10K_003441
source
No Value

T7 RNA polymerase defined as a CE part. T7 cannot function with C terminal fusions, so that option is taken away with this part. Has not been successfully synthesized.

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … AGGT - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-CE_T7RNApol_Stop
plasmid size
4776
insert size
2652
origin
ColE1
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.