H3H

hispidin-3-hydroxylase
BBF10K_003487
source
Neonothopanus nambi

converts hispidin into luciferin. Required as second step in fungal bioluminescence pathway

attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 for
reverse primer
M13 rev

Construct

plasmid name
pOpen-H3H
plasmid size
3392
insert size
1269
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

No Value