HispS_1

hispidin synthase
BBF10K_003488
source
Neonothopanus nambi

converts caffeic acid to hispidin. Required as first step in fungal bioluminescence pathway. NOTE: this gene was split because it was too large. Both fragments are required for cloning

attr.
Andreas Stürmer, Sebastian Cocioba, Justin Atkin

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
N/A
promoter
N/A
inducer
N/A

cloning

method
GoldenGate
enzyme
BsaI
overhangs
3' - AATG … GCTT - 5'

sequencing

forward primer
M13 for
reverse primer
M13 rev

Construct

plasmid name
pOpen-HispS_1
plasmid size
5400
insert size
3277
origin
ColE1 High Copy
copy number
500-700

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
No Value
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.