T7_Orf1A_1

SARS-CoV-2 Orf1A positive control
BBF10K_003505
source
SARS-CoV-2

PolT7-driven expression of a 508nt part of the Sars-CoV-2 OrfA RNA, to be used as control

attr.
Open Bioeconomy Lab

Usage

growth

shipping strain
Escherichia coli Top10
growth conditions
37 C, shaking 300 rpm
antibiotic
Ampicillin

expression

strain
Escherichia coli
promoter
T7
inducer
LacIq

cloning

method
N/A
enzyme
N/A
overhangs
3' - N/A … N/A - 5'

sequencing

forward primer
M13 For
reverse primer
M13 Rev

Construct

plasmid name
pOpen-T7_Orf1A_1
plasmid size
2665
insert size
664
origin
ColE1
copy number
high (3-500)

Safety

BSL
BSL1

other information

No Value

References

primary references

NCBI
UniProt
Available Elsewhere
FALSE

intellectual property

We are unaware of third-party property rights claims on uses of this item.